Cyclooxygenase-2 network as predictive molecular marker for clinical pregnancy in in vitro fertilization.
Infect. Immun. 2010 Dec 13. Xiao J, Jones-Brando L, Talbot CC Jr, Yolken RH; The Stanley Division of Developmental Neurovirology, Institute for Basic Biomedical Sciences, Johns Hopkins School of Medicine, Baltimore, MD, USA
“The receptivity of the endometrium for an intruding blastocyst is an important factor in the implantation of a human embryo in the uterus. The goal of our study was to establish molecular markers for endometrial receptivity and clinical pregnancy in stimulated cycles during in vitro fertilization (IVF) treatment.
IPA showed us that the differentially expressed genes between pregnant and non-pregnant patients from our microarray experiments were connected into one single network with cyclooxygenase-2 (COX-2 or PTGS2) in a central role. Ingenuity saved us time and effort and provided us with information that we could not have found with a classic literature search. Ingenuity’s software was definitely a step forward in our quest towards a genetic signature for endometrial receptivity in stimulated cycles for IVF."
Inge Van Vaerenbergh, MS and Claire Bourgain, MD, PhD
Department of Pathology, University Hospital Brussels, Belgium
Reproductive Immunology and Implantation, VUB, Belgium
Fertility and Sterility, January 2011
Inge Van Vaerenbergh, Christophe Blockeel, Leentje Van Lommel, Vanessa Ghislain, Peter In't Veld, Frans Schuit, Human Mousavi Fatemi, Paul Devroey, Claire Bourgain;
Department of Pathology, Vrije Universiteit Brussel and UZ Brussel, Jette, Belgium; Centre for Reproductive Medicine, UZ Brussel, Jette, Belgium; Gene Expression Unit, Department of Molecular Cell Biology, KU Leuven, Leuven, Belgium; Royale Hayat Hospital, Kuwait
This month we feature research that was published in Fertility and Sterility and presented as a poster at the February 2011 Molecular Medicine Tri-Conference in San Francisco. These researchers examined the gene expression of stimulated IVF cycle human endometrium on the day of oocyte retrieval of pregnant and nonpregnant patients, and compared this to two independent groups with different GnRH-antagonist ovarian stimulation protocols. They utilized IPA to help them discover molecular markers for endometrial receptivity in in vitro fertilization cycles, specifically with COX-2/PTGS2 pathways. The data suggests that on the day of oocyte retrieval in GnRH-antagonist cycles, increased gene expression of cyclooxygenase-2 (COX-2), together with the expression of other molecules in the cyclooxygenase-2 network, coincides with a lower probability of achieving clinical pregnancy in this cycle. |
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