Publication Synopsis

Human mucosal in vivo transcriptome responses to three lactobacilli indicate how probiotics may modulate human cellular pathways.

"Biological interpretation of transcriptomes from human tissue biopsies containing multiple cell types, each type with its own expression program, is notoriously difficult. With gene expression data from human mucosal intestinal biopsies as input, we used IPA to confidently reconstruct and integrate cellular regulatory networks and canonical pathways via the use of IPA's cell- and tissue-specific filters. Annotating these pathways and networks with detailed contextual information from Ingenuity's drug and disease databases, we evaluated differential expression profiles of human mucosal tissues after consumption of preparations of three different species of probiotic lactic acid bacteria for effects on human mucosa. Using IPA's integrative features, we obtained leads to mucosal gene expression profiles that appear to be uniquely associated with each individual species of lactobacilli, leads that will enable us to focus our validation studies."

Dr. Peter van Baarlen, Ph.D.
Assistant Professor at the Host-Microbe Interactomics Group
Wageningen University, the Netherlands