A Combined Biological and Bioinformatic Analysis of Primary and Metastatic Tissues from NGS Ewing’s Sarcoma Patients
The Ewing’s Sarcoma family of tumors is a category of cancers that predominantly affects teenagers between the ages of 10 to 20. Learn how QIAGEN Silicon Valley’ IPA software and Integromics’ SeqSolve software were used to investigate Ewing’s Sarcoma patient samples generated from Helicos’ NGS technology. We will present a combined bioinformatic and biological analysis of Ewing’s Sarcoma patient samples, focusing on the differences between primary and metastatic tissues. IPA was used to analyze the significantly regulated genes and Integromics’ NGS SeqSolve software was used to prepare the RNA-Seq data. IPA’s new transcription factor analysis tool and downstream effects map were used to help narrow down targets and visualize the biological networks.