Explore Ingenuity Webinars and learn how Ingenuity products can answer your scientific questions

John A. Martignetti Headshot
Garry Nolan Ph.D. Headshot

Realizing the promise of genomics through rapid innovation in biological analysis and interpretation

Presenter: John A. Martignetti, M.D., Ph.D. and Garry Nolan Ph.D.
Original Broadcast Date:Thursday, March 13, 2014

Discover how intuitive web-based applications can help scientists quickly analyze and accurately interpret the biological meaning in their genomic data.

In the last few years, researchers and clinicians have been faced with the challenge of how to effectively sort through and accurately understand the biological meaning from their genomic data. Now, web-based applications such as QIAGEN's Ingenuity Platform can be used to better comprehend complex biological systems, answer questions, analyze and interpret data.

In this webinar, the audience will discover how Ingenuity Pathway Analysis (IPA) and Ingenuity Variant Analysis softwares can be used to provide new insight into and quick interpretation of their scientific findings.

The speakers, John Martignetti and Garry Nolan, will discuss how these software solutions were applied to their respective research areas to more effectively search, explore, visualize, analyze and interpret biological findings related to genes, proteins and small molecules.

Vivien Sheehan Headshot

Using QIAGEN’s Ingenuity Variant Analysis in Pharmacogenomics

Presenter: Vivien Sheehan, Asst. Prof. of Pediatrics Baylor College of Medicine
Original Broadcast Date: Thursday December 5, 2013

Vivien Sheehan will be speaking about her work in pharmacogenomics. This presentation will cover sickle cell disease (SCD), specifically the genetic component to fetal hemoglobin response to hydroxyurea. Such knowledge will enhance the ability to predict drug response, improving patient care by allowing selection of individuals who will benefit most from hydroxyurea, and offer alternative therapies.

Variant Analysis Tips and Tricks[34:11 minutes]
Presented by Dr. Darryl Gietzen, Senior Field Applications Scientist, QIAGEN Redwood City (formally Ingenuity Systems, Inc.)

Whether you are a new or experienced user of Ingenuity Variant Analysis, you will benefit from attending this Tips and Tricks presentation. This presentation will walk you through several scenarios and give tips on how to work more efficiently and get more accurate scientific results. These tips come from the people who work with Variant Analysis every day: the Field Application Scientist and Customer Support teams.

In this webinar you will learn fast and efficient variant analysis design and set-up (covering several study types and sample numbers), how to quickly re-analyze the same samples with different filter settings, how to get the most from the Genetic Analysis filter module without over filtering, optimization of the filter cascade, and quickly prioritize biologically compelling variants.

2013 IPA Fall Release Product Introduction Webinar [34:11 minutes]
Presented by Dr. Stuart Tugendreich, Scientific Director, IPA
See the whole picture! Powerful functionality enables you to understand causal connections between molecules and diseases. This Fall 2103 release of QIAGEN’s Ingenuity Pathway Analysis (IPA) has exciting new capabilities which enable interactive visual exploration of causality between molecules and disease, functions, or phenotypes.
Life Long Changes in DNA Methylation & ncRNAs in Fetal Alcohol Syndrome (FAS) [54:54 minutes]
Presented By: Ben Laufer, PhD Candidate, Western University, Ontario, Canada
Fetal alcohol spectrum disorders (FASDs) are characterized by life-long changes in gene expression, neurodevelopment and behavior. What mechanisms initiate and maintain these changes are not known, but current research suggests a role for alcohol-induced epigenetic changes. We assessed alterations to adult mouse brain tissue by assaying DNA cytosine methylation and small noncoding RNA (ncRNA) expression, specifically the microRNA (miRNA) and small nucleolar RNA (snoRNA) subtypes. We found long-lasting alterations in DNA methylation as a result of fetal alcohol exposure, specifically in the imprinted regions of the genome harboring ncRNAs and sequences interacting with regulatory proteins. The findings of this study help to expand on the mechanisms behind the long-lasting changes in the brain transcriptome of FASD individuals.
Leveraging QIAGEN’s Ingenuity for Predictive Systems Biology: An Approach To Broad-Spectrum, Host-Directed Drug Target Discovery In Infectious Diseases [50:33 minutes]
Presented By: Dr. Ramon Felciano, Co-founder and Senior Vice President, Applied Research and Partnering, QIAGEN Redwood City
Knowledge of immune system and host-pathogen pathways can inform development of targeted therapies and molecular diagnostics based on a mechanistic understanding of disease pathogenesis and the host response. We used QIAGEN’s Ingenuity platform to investigate the feasibility of rapid target discovery for novel broad-spectrum molecular therapeutics was investigated through comprehensive systems biology modeling and analysis of pathogen and host-response pathways and mechanisms. We used this approach to identify and prioritize candidate host targets based on strength of mechanistic evidence characterizing the role of the target in pathogenesis and tractability desiderata that include optimal delivery of new indications through potential repurposing of existing compounds or therapeutics. We will describe our approach, experimental results, and the key technology innovations now publically available in IPA.
IPA the Fast Path to Toxicity Targets of Interest [36:55 minutes]
Presented by Dr. Aaron Erdely, Health Effects Laboratory Division, NIOSH
and Kaushal Parekh, Associate Staff Ontology Engineer, QIAGEN Redwood City
Metal-rich particulate matter inhalation exposure results in target organ toxicity but also adverse systemic effects including cardiovascular dysfunction and immunosuppression. As a preliminary search for induction of systemic mechanisms, generation of comprehensive transcriptome datasets by DNA microarray, along with gene network analysis, was performed from circulating whole blood cells, aorta, and lung then compared to determine related biological signaling following inhalation exposure.
Also demonstrated are some exciting new features in the new 2013 IPA Spring Release. Learn how IPA can help you quickly filter down to specific toxicity targets of interest
IPA 2013 Spring Release [48:24 minutes]
Presented by Dr. Stuart Tugendreich, Scientific Director, IPA
The 2013 IPA Spring Release is here! Powerful new functionality enables you to upload, find, and compare datasets, and understand causal connections between diseases, genes, and networks of upstream regulators. Stuart Tugendriech, PhD, Scientific Director, IPA from QIAGEN Redwood City gives an overview of the new IPA capabilities in the release, as well as a use case utilizing the new features and how IPA helps to Discover Causal Connections. Faster.
Improving Single Genome Annotation by Multi-Genome Analysis [37:32 minutes]
Presented by Gustavo Glusman, Senior Research Scientist, Institute for Systems Biology
This webinar discusses the analysis of whole genome sequence data in Alternating Hemiplegia of Childhood (ACH) samples using custom workflows and QIAGEN’s Ingenuity Variant Analysis platform to improve the sensitivity and specificity of genome interpretation.
Knowledge-Based Tools for Comprehensive Interpretation of Variant & Gene Expression Data [56:16 minutes]
Presented by Jean-Noel Billaud and Megan Laurance
Staff Scientists Jean-Noel Billaud and Megan Laurance present strategies for integrated analysis and interpretation of variant and gene expression data generated from cell lines representative of 2 breast cancer subtypes: Claudin-Low and Luminal. These subtypes represent different disease entities associated with specific molecular alterations and histo-clinical features. Interrogating these samples at both the variant and transcript level with Ingenuity's Variant Analysis and IPA software presents a powerful approach to drawing clear molecular paths from variants and gene expression changes to phenotypes relevant to these disease subtypes including Epithelial-to-Mesenchymal Transition and Metastasis.
A Bioinformatician's Guide to Lung Cancer: Wnt7a Signaling and Beyond [40:13 minutes]
Presented by Dr. Walter Bottje, Professor, Dept. of Poultry Science, University of Arkansas
This webinar discusses an important antitumor pathway in lung cancer (Wnt7a signaling) as a framework to explain the bioinformatic analysis process using IPA. Topics covered include biological function and pathway analysis, network construction, and identifying and interpreting upstream regulators.
Differential Expression of Focus Genes Associated Feed Efficiency [41:26 minutes]
Presented by Dr. Walter Bottje, Professor, Dept. of Poultry Science, University of Arkansas
Global RNA expression in breast muscle obtained from a male broiler line phenotyped for high or low feed efficiency (FE) was investigated using microarray analysis. By using an overlay function of IPA in which canonical pathways can be projected onto a set of genes, differentially expressed focus genes were identified. We selected 130 out of 260 possible canonical pathways in the IPA program that would likely be associated with normal metabolic activities and did not select those that were obviously tissue or disease specific. The results of this study provide additional insight into gene expression in muscle associated with the phenotypic expression of feed efficiency in broilers.
The Role of microRNAs in Kidneys of Hypertensive Patients [23:09 minutes]
Presented by Aimee Jackson, Ph.D.
microRNAs are small, non-coding RNAs that function as central regulators of gene expression and development. These regulatory molecules have been implicated in a wide range of normal and pathological activities, including embryonic development, cancer, inflammation, cardiovascular disease and viral infections. We explored the possibility that microRNA dysfunction in the kidney might contribute to hypertension, a significant health issue. We analyzed mRNA and microRNA expression profiling data from kidneys of untreated hypertensive patients and normotensive patients to identify microRNAs, microRNA targets, and gene networks that are dysregulated in hypertension. The results of these analyses identify microRNAs and their targets that could be biomarkers or therapeutic targets for hypertension.