The fastest way to understand the biological meaning of your expression data

Rapidly Identify Biologically Compelling Transcript Changes

QIAGEN® Ingenuity iReport is an interactive web-based report, optimized for gene expression experiments from – RNA-Seq, microarray, and real-time PCR platforms, that provides a fast and accurate biological and statistical analysis of your experimental data.

Indentify and Prioritize Variants Graphic - Ingenuity Ireport
interactive
Interactive
Visually organize and filter results to identify biologically compelling, functionally relevant gene expression changes
pay per sample
Easy To Use And Purchase
Spend your time focused on biology, not on training, running statistics, or software licensing
accurate
Accurate
Based on evidence-backed insights from the Ingenuity® Knowledge Base

Ingenuity iReport for Human Isoform-level RNA-Seq Data

expression pipeline
Quick Identification
Quickly identify genes that have multiple differentially expressed isoforms
the wheel
Visualize Data
Visualize your data in the context of isoform view
Targeted Bibliographies
Targeted Bibliographies
Compile targeted bibliographies with functional information on specific isoforms
Starting with FASTQ files? We’ve partnered with Maverix Biomics
Maverix icon

The Ingenuity Knowledge Base

knowledge base graphic

All of QIAGEN's Ingenuity Product Line solutions leverage the Ingenuity Knowledge Base, a repository of expertly curated biological interactions and functional annotations created from millions of individually modeled relationships between proteins, genes, complexes, cells, tissues, drugs, and diseases. These modeled relationships, or Findings, include rich details, links to the original article, and are reviewed for accuracy by PhD scientists. The curated content in the Knowledge Base is structured into an Ontology that allows for contextual information, computation by the applications, and synonym resolution to ensure consistency across concepts. These features make the Ingenuity Knowledge Base distinctive and unparalleled to any other database.

Ingenuity iReport has a broad set of features that allow you to quickly understand and visualize your data

Differential Expression Pipeline

The Ingenuity iReport Data Analysis Pipeline incorporates best practices in statistical, quality, and differential expression analysis of gene expression data. The Ingenuity iReport data analysis pipeline is written in the R statistical programming language and makes extensive use of the Bioconductor project; a widely used and frequently cited collection of tools for the analysis of high-throughput genomic data.

Free Data Analysis and Ingenuity iReport Preview

It’s easy to get started in Ingenuity iReport. Upload your data through QIAGEN’s secure site, and receive a complete analysis of your gene expression data plus a preview of the Pathways, Processes, and Diseases relevant to your differentially expressed genes. The Ingenuity iReport Preview gives you the key information you need in order to make an informed purchasing decision. Previews also enable you to request any changes to the data analysis parameters.

Enrichment for Pathways, Biological Processes, and Diseases

Ingenuity iReport uses the content in the Ingenuity Knowledge Base and analytics such as the Fisher's exact test to perform an annotation enrichment analysis which identifies Pathways, Biological Processes, and Diseases that are significantly overrepresented in your differentially expressed genes. Ingenuity iReport enables visualization of your genes in the context of those biological processes. Evidence from the biomedical literature substantiating the role of your genes in those biological processes is at your fingertips.

Gene to Disease Associations

Ingenuity iReport goes beyond simple associations between genes and disease annotations and further classifies these relationships based on Biomarker, Drug Target, Gene Expression, and Mutation evidence from the biomedical literature, as well as trusted databases such as clinicaltrials.gov and the Mouse Genome Database.

Molecular Interactions

Ingenuity iReport identifies molecular connections between your differentially expressed genes, enabling visualization of hubs of regulation (expression, activation, post-translational modification) and physical interaction (protein:DNA, protein:protein binding).

Isoform-level analysis of human RNA-Seq data

Isoform Ingenuity iReport identifies isoforms that are differentially expressed between condition and control samples, and interprets the impact of those expression changes in the context of Isoform View, which displays alternatively spliced transcripts and details of their chromosomal locus, predicted protein domains, and encoded protein isoforms.

The Wheel

Browse your entire Ingenuity iReport starting with whatever topic is most relevant to your study: a specific gene, biological process, cellular phenotype, or pathway. Use visual cues to group genes based on molecular function, pathway membership, subcellular location, or hubs of molecular interaction to find compelling clusters of gene expression changes.

Create and Export Topic-Specific Bibliographies

The Publications and Findings comprise the evidence substantiating the role of your differentially expressed genes in pathways, phenotypes, diseases and molecular interactions. Lists of key publications are easy to compile and export from Ingenuity iReport. Export these targeted bibliographies into End Note format to integrate insights from iReport into existing reference databases.

Unlimited Sharing

It’s very easy to Share your Ingenuity iReport with colleagues and collaborators. Enter their e-mail into the Share field and they will gain full access to the data analysis and biological insights in Ingenuity iReport.

Personalized Tour of your Ingenuity iReport

Get up to speed quickly on how Ingenuity iReport works, and some key insights from your data by taking The Tour, which accompanies every Ingenuity iReport.

Explore Our Webinars and see how the scientific community has used Ingenuity iReport to create novel discoveries

Analysis of NSCLC adenocarcinomas with and without KRAS mutations using Ingenuity iReport [50:51 minutes]
Presented by Megan Laurance, Ph.D., QIAGEN
QIAGEN presents an analysis of lung adenocarcinomas with and without KRAS mutations in Ingenuity iReport and provides a demonstration of the statistical analysis and biological interpretation. The analysis strategies presented will be applied to RNA-Seq data made publicly available by Dr. Krishna Kalari at the Mayo Clinic. The webinar will be presented by Dr. Megan Laurance, Staff Scientist at QIAGEN.
Strategies for Identifying Biologically Compelling Genes from Breast Cancer Subtype RNA-Seq Profiles [49:10 minutes]
Presented by Megan Laurance, Ph.D., QIAGEN
Megan demonstrates analysis strategies and best practices for identifying the most biologically-relevant genes from expression profiles of breast cancer cell lines. The analysis utilizes new tools and workflows in Ingenuity iReport to help researchers rapidly identify subsets of differentially expressed genes connected to the functional endpoints and phenotypes most relevant to breast cancer subtypes, with an aim of identifying genes to take back to the lab for validation studies. The analysis strategies presented in this webinar can be applied to a variety of gene expression datasets, including RNA-Seq and microarray experiments.
Gene Expression Analysis Using Ingenuity iReport to Analyze Response to H1N1 Influenza [49:58 minutes]
Presented by Jamie L. Fornak, Ph.D. MPH
This webinar uses previously published data from an influenza study to demonstrate the ability of Ingenuity iReport to correctly identify expected results and gain additional novel biological insights from gene expression data. Lee et al. originally performed gene expression analysis on human type-I like alveolar epithelial cells that were mock infected or infected with seasonal H1N1or pandemic H1N1. Two separate Ingenuity iReport analyses were generated from this dataset: one comparing mock infection to seasonal H1N1 infection and another comparing mock infection to pandemic H1N1 infection. See how Ingenuity iReport expanded the insights described in the original publication and provided additional genes of interest for potential follow up studies in the areas of transcription and mRNA transport.
Understanding Maternal Hypercholesterolemia Effect on Offspring: Microarray Data Analysis with Ingenuity iReport [1:05:35 minutes]
Presented by Megan Laurance, Ph.D., QIAGEN
This webinar will introduce Ingenuity iReport and demonstrate how it can be used to get a rapid and accurate biological understanding of expression data coming straight off the machines, without using intermediary statistical software or bioinformatics tools. The example used is a cardiovascular dataset and we'll demonstrate how Ingenuity iReport can quickly help you identify genes and gene networks implicated in observed phenotypical changes.